LOCUS       40924_4739        5485 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pARG1K DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pARG1K
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5485)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5485)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    241..894
                     /pseudo
                     /gene="arg1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             241..894
                     /pseudo
                     /codon_start=1
                     /gene="arg1"
                     /label=arg1
                     /note="NotI site is created at the middle of this fragment 
                     (551-558)."
                     /translation="SRSC*R*GLLFV*QGRS*IH*LHLWRSCYIFGSCSS*SC*TCR*S
                     MLQISP**QPFLQRACH*AI*CH**FFGKK*RYCWPHKNFLCQLWY*GQ*DSLEVRS*G
                     GRV*EVWRREESNCLL**LFPRPFFGIS*YHCEPQI*TWFPTITS*CRPSRL**PCIHR
                     AICQ*QNCGCYCRARSR*RWYLSCQA*IFDCFAQGL**GWCFFNL**NSMRTWSQ"
     gene            241..894
                     /pseudo
                     /gene="arg1"
                     /label=arg1
     gene            938..2294
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     complement(2345..2361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2574..3029
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3311..3415
                     /label=AmpR promoter
     CDS             3416..4273
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4447..5035
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5323..5344
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5359..5389
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5397..5413
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5421..5437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtcgtagct gctaaaggtg agggctccta tttgtttgac aaggaaggtc gtaaatacat
      301 tgacttcacc tctggcgtag ctgttacatc tttgggtcat gctcatcctg aagttgctag
      361 acttgccgct gatcaatgct ccaaattagt ccatagtagc aaccttttct acaacgagcc
      421 tgccattgag ctatctaatg tcattaataa ttctttggca aaaaatagcg gtattgctgg
      481 ccccacaaaa attttctttg ccaattgtgg tactgaggcc aatgagacag ccttgaagtt
      541 cgctcgtaag gcggccgcgt ttgagaagta tggagaaggg aagagtcaaa ttgtttactt
      601 taataactct ttccacggcc gttctttggg atctcttagt atcactgcga accccaaata
      661 taaacgtggt ttccaaccat tacttcctga tgtcgtccaa gccgtctata atgaccctgc
      721 atccatagag caatttgtca atgacaaaac tgcggctgtt attgtcgagc ccgttcaagg
      781 tgaaggtggt atttgtcctg ccaagcctga atttttgatt gctttgcgca aggcctgtga
      841 taaggttggt gcttctttaa tctatgatga aattcaatgc ggacttggtc gcagatctgt
      901 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
      961 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     1021 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     1081 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     1141 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     1201 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     1261 cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag gccgcgatta
     1321 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1381 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1441 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1501 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1561 ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat
     1621 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1681 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1741 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1801 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     1861 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1921 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1981 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2041 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagtactg
     2101 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     2161 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
     2221 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     2281 tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtttaa acgagctcga
     2341 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2401 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2461 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     2521 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     2581 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2641 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2701 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2761 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     2821 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2881 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     2941 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3001 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     3061 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3121 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3181 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3241 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3301 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3361 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3421 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3481 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3541 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3601 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3661 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3721 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3781 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3841 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3901 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3961 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4021 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4081 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4141 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4201 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4261 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4321 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4381 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4441 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4501 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4561 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4621 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4681 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4741 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4801 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4861 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4921 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4981 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5041 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5101 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5161 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     5221 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     5281 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     5341 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     5401 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg
     5461 cctgcaggtc gactctagag gatcc
//
